| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Integrin alpha-L;CD11 antigen-like family member A;Leukocyte adhesion glycoprotein LFA-1 alpha chain;LFA-1A;Leukocyte function-associated molecule 1 alpha chain;CD11a;ITGAL;CD11A; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CD11a recombinant protein (Position: F161-L349). Human CD11a shares 73.7% mino acid (aa) sequence identity with mouse CD11a. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ITGAL (Integrin alpha-L) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CD11a/ITGAL Antibody Picoband® catalog # A04466-1. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human CD11a recombinant protein (Position: F161-L349). Human CD11a shares 73.7% mino acid (aa) sequence identity with mouse CD11a. (reported region: F161-L349).
- Molecular weight context: reported MW: 150 kDa; calculated MW: 128770 MW
- Reactivity: Human
- Applications: Flow Cytometry, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Integrin alpha-L; Integrin alpha-L. CD11a, also known as ITGAL or LFA-1, is a human gene which functions in the immune system. It is mapped to 16p11.2. CD11a is involved in cellular adhesion and costimulatory signaling. It is the target of the drug efalizumab. CD11a encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. It is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1. Functional note: Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Reported localization: Membrane; Single-pass type I membrane protein. Expression/tissue context: Leukocytes.
Research relevance and current trends
- Cell Adhesion: Researchers commonly examine how ITGAL (Integrin alpha-L) relates to this theme using model systems and orthogonal readouts.
- Cell Type Markers: Researchers commonly examine how ITGAL (Integrin alpha-L) relates to this theme using model systems and orthogonal readouts.
- Cytoskeleton/ECM: Researchers commonly examine how ITGAL (Integrin alpha-L) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ITGAL (Integrin alpha-L) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of ITGAL (Integrin alpha-L) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
