| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin alpha-L;CD11 antigen-like family member A;Leukocyte adhesion glycoprotein LFA-1 alpha chain;LFA-1A;Leukocyte function-associated molecule 1 alpha chain;CD11a;ITGAL;CD11A; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD11a recombinant protein (Position: F161-L349). Human CD11a shares 73.7% mino acid (aa) sequence identity with mouse CD11a. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects integrin alpha-L (gene ITGAL) in human samples and is validated for Western blot. The predicted molecular weight is 128.8 kDa, and the supplier reports an observed band at about 180 kDa.
Integrin alpha-L (ITGAL, CD11a) pairs with integrin beta-2 to form LFA-1, a leukocyte integrin that binds ICAM-1 and other ICAM family members. LFA-1 mediates leukocyte adhesion to endothelium, the immunological synapse between T cells and antigen-presenting cells, and cytotoxic cell killing.
| Target | Integrin alpha-L (gene ITGAL; UniProt P20701, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 128.8 kDa |
| Observed MW | 180 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human Jurkat cells, human THP-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human CD11a fragment (Phe161–Leu349), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places integrin alpha-L in the cell membrane and membrane raft. Tissue expression noted by UniProt (human): Leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huiping Wang et al. (2006) Protective effect of bicyclol on acute hepatic failure induced by lipopolysaccharide and d-galactosamine in mice. European Journal of Pharmacology. 10.1016/j.ejphar.2005.12.080
Lingmin Mu et al. (2014) Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy. Iranian Journal of Basic Medical Sciences. PMID: 25691929
