| Field | Specification |
|---|---|
| Target | |
| Alternative names | Prominin-1; Antigen AC133; Prominin-like protein 1; CD antigen CD133; PROM1; PROML1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD133 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27P09 is a rabbit monoclonal antibody that recognizes prominin-1 (gene PROM1) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 97.2 kDa, and the supplier reports an observed band at about 115 kDa.
Prominin-1 (PROM1, CD133) is a pentaspan membrane glycoprotein concentrated in plasma membrane protrusions such as microvilli and cilia, where it binds cholesterol. It is a widely used surface marker of hematopoietic and neural stem cells and of cells with stem-like properties in several tumors, and it is required for photoreceptor disk formation.
| Target | Prominin-1 (gene PROM1; UniProt O43490, human) |
|---|---|
| Clone | 27P09 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 97.2 kDa |
| Observed MW | 115 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human CD133.
Reactivity Notes
The supplier lists reactivity with human. UniProt places prominin-1 in the apical cell membrane, cell projection and endoplasmic reticulum. Tissue expression noted by UniProt (human): Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yong Li et al. (2017) Stanniocalcin-1 augments stem-like traits of glioblastoma cells through binding and activating NOTCH1. Cancer Letters. 10.1016/j.canlet.2017.11.033
Te Liu et al. (2010) Characterization of primary ovarian cancer cells in different culture systems. Oncology Reports. 10.3892/or_00000761
