| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tumor necrosis factor ligand superfamily member 8;CD30 ligand;CD30-L;CD153;TNFSF8;CD30L, CD30LG; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CD153 recombinant protein (Position: Q63-D234). Human CD153 shares 75% amino acid (aa) sequence identity with mouse CD153. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of TNFSF8 (Tumor necrosis factor ligand superfamily member 8) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CD153/TNFSF8 Antibody catalog # RP1103. Tested in Flow Cytometry, IHC applications. This antibody reacts with Human.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human CD153 recombinant protein (Position: Q63-D234). Human CD153 shares 75% amino acid (aa) sequence identity with mouse CD153. (reported region: Q63-D234).
- Molecular weight context: reported MW: 86 kDa; calculated MW: 26017 MW
- Reactivity: Human
- Applications: Flow Cytometry, IHC
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Tumor necrosis factor ligand superfamily member 8; Tumor necrosis factor ligand superfamily member 8. CD153 is a ligand for CD30. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene. Functional note: Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. Reported localization: Membrane; Single-pass type II membrane protein. Expression/tissue context: Widely expressed.
Research relevance and current trends
- Adaptive Immunity: Researchers commonly examine how TNFSF8 (Tumor necrosis factor ligand superfamily member 8) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how TNFSF8 (Tumor necrosis factor ligand superfamily member 8) relates to this theme using model systems and orthogonal readouts.
- T Cells: Researchers commonly examine how TNFSF8 (Tumor necrosis factor ligand superfamily member 8) relates to this theme using model systems and orthogonal readouts.
Common research applications
- IHC/IHC-F: assess spatial distribution of TNFSF8 (Tumor necrosis factor ligand superfamily member 8) across tissue regions and cell types using matched controls.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
