| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor ligand superfamily member 8;CD30 ligand;CD30-L;CD153;TNFSF8;CD30L, CD30LG; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD153 recombinant protein (Position: Q63-D234). Human CD153 shares 75% amino acid (aa) sequence identity with mouse CD153. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes tumor necrosis factor ligand superfamily member 8 (gene TNFSF8) in human samples and is validated for flow cytometry and IHC. The predicted molecular weight is 26.0 kDa.
CD30 ligand (CD30L, TNFSF8, CD153) is a TNF family cytokine that binds the receptor CD30 (TNFRSF8). It is expressed on activated T cells and other immune cells, can drive T-cell proliferation, and is studied in lymphocyte activation, immune regulation and CD30-positive lymphomas.
| Target | Tumor necrosis factor ligand superfamily member 8 (gene TNFSF8; UniProt P32971, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 26.0 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL |
|---|---|
| Flow cytometry | 1–3 µg/1×106cells |
Samples with confirmed staining (IHC): human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with a confirmed signal (flow cytometry): U937 cells.
Immunogen
Recombinant human CD153 fragment (Gln63–Asp234), expressed in E. coli. Sequence identity with the mouse ortholog: 75%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places tumor necrosis factor ligand superfamily member 8 in the membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
