| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin beta-2;Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta;Complement receptor C3 subunit beta;CD18;ITGB2;CD18, MFI7; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD18 recombinant protein (Position: Q404-S769). Human CD18 shares 76% amino acid (aa) sequence identity with mouse CD18. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes integrin beta-2 (gene ITGB2) in human samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 84.8 kDa, and the supplier reports an observed band at about 90–100 kDa.
Integrin beta-2 (CD18) is the shared beta subunit of the four leukocyte β2 integrins, including LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). These receptors bind ICAMs, complement fragment iC3b and fibrinogen and mediate leukocyte adhesion, migration into tissues and phagocytosis; loss of CD18 causes leukocyte adhesion deficiency type 1.
| Target | Integrin beta-2 (gene ITGB2; UniProt P05107, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 84.8 kDa |
| Observed MW | 90–100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human Jurkat cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human tonsil tissue.
Immunogen
Recombinant human CD18 fragment (Gln404–Ser769), expressed in E. coli. Sequence identity with the mouse ortholog: 76%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places integrin beta-2 in the cell membrane and membrane raft. Tissue expression noted by UniProt (human): Leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jie Li et al. (2025) Fibrin induces infiltration of macrophages and neutrophils via integrin αMβ2 and triggers aortic dissection. British Journal of Pharmacology. 10.1111/bph.70033
Kotaro Horiguchi et al. (2016) Identification of THY1 as a novel thyrotrope marker and THY1 antibody-mediated thyrotrope isolation in the rat anterior pituitary gland. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2016.10.049
