| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Host | |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Shipping | |
| Species | |
| Storage | |
| Target | |
| UniProt # |
Immunotherapy
Product Overview
This purified recombinant monoclonal antibody is a human Anti-CD19 IgG which recognizes CD19 antigen. The variable regions for this antibody are based on an FDA-approved drug. This antibody has been tested for specific binding affinity to purified human CD19 protein (BPS Bioscience, #101015) in a colorimetric ELISA binding assay.
| Clonality | Monoclonal |
|---|---|
| Host | Human |
| Antibody Type | IgG1 |
| Isotype | IgG1 |
| Reactivity | Human |
| Purification | Protein A affinity purification (HEK293) |
| Purity | ≥90% |
| Molecular Weight | HC: ~49.9 kDa; LC: ~23.9 kDa |
| Formulation | 8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol |
| UniProt | P15391 |
| Storage | Aqueous buffer solution. |
Validated Applications
- Western Blot
Assay conditions: Purified human his-tagged CD19 was bound to clear 96-well nickel plate overnight in 4°C (1 µg/mL). Upon washing and blocking wells for 1 hour at room temp, serial dilutions of anti-CD19 (200 nM to 0nM in 3-fold dilutions) were incubated in each well for 1 hr at room temp (slow shaking). Next, wells were washed and incubated with anti-human IgG-HRP for 1 hour at room temp (slow shaking) then washed again. For detection, the wells were incubated with Colorimetric HRP Substrate (BPS Bioscience #79651) for 1-10 minutes until a blue color developed in the positive control. The reaction was then quenched with equal volume of 1N HCL and absorbance read at 450 nm.
Immunogen / Antigen Information
Immunogen information not provided. Please refer to the product datasheet for detailed antigen information.
Cross-Reactivity & Specificity
Cross-reactivity data: Species reactivity as listed. No additional cross-reactivity data is available from the manufacturer.
Safety & Handling
Avoid freeze/thaw cycles.
For Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle with standard laboratory precautions (gloves, safety glasses, lab coat). Avoid freeze-thaw cycles. Consult SDS prior to use.
Related Products
- 100732
- 100761
- 79651
- 101015
This antibody has been validated for: Western Blot. Optimal dilutions and concentrations should be empirically determined for each specific application and sample type. Refer to the product datasheet for suggested starting conditions.
This antibody should be stored at the indicated temperature to maintain stability. Store at Aqueous buffer solution.. Avoid repeated freeze-thaw cycles, which can reduce antibody performance and increase aggregation. Centrifuge briefly after thawing and keep on ice during use.
Please refer to the BPS Bioscience datasheet for detailed immunogen information.
Cross-reactivity data is limited; refer to the product datasheet for available information.
BPS Bioscience offers multiple antibody formats for key checkpoint and immunotherapy targets. Contact BioHippo (support@biohippo.com) or click "Talk to a Scientist" to inquire about additional conjugate formats, bulk quantities, or custom formulations.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
