| Field | Specification |
|---|---|
| Target | |
| Alternative names | Antigen-presenting glycoprotein CD1d; R3G1; CD1d; CD1D |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD1D recombinant protein (Position: E20-W295). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets antigen-presenting glycoprotein CD1d in human and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 37.7 kDa, and the supplier reports an observed band at about 55 kDa.
CD1d is a non-classical MHC class I-like molecule that presents glycolipid antigens, such as α-galactosylceramide, to invariant natural killer T (iNKT) cells. It is expressed on antigen-presenting cells and some epithelial cells, and it is central to studies of NKT-cell biology and lipid-driven immune regulation.
| Target | Antigen-presenting glycoprotein CD1d (gene CD1D; UniProt P15813, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 37.7 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat spleen tissue, rat lung tissue, rat heart tissue, human THP-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Recombinant human CD1D fragment (Glu20–Trp295), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places antigen-presenting glycoprotein CD1d in the cell membrane, basolateral cell membrane, endosome membrane and lysosome membrane. Tissue expression noted by UniProt (human): Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
