| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Antigen-presenting glycoprotein CD1d; R3G1; CD1d; CD1D |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CD1D recombinant protein (Position: E20-W295). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CD1D Antibody Picoband® is an antibody for CD1D detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: CD1D (CD1d molecule); UniProt: P15813
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 55 kDa, calculated 29041 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-CD1D Antibody Picoband® catalog # A00323.
Biological background
Biological context: Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
Expression and localization notes: cellular localization: Endoplasmic reticulum membrane; Single-pass type I membrane protein; Cell membrane; Basolateral cell membrane; Lysosome membrane; Endosome membrane, tissue context: Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues..
Common research applications
- Western blotting (WB): Compare CD1D levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of CD1D in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify CD1D-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: CD1D (CD1d molecule) also known as R3G1, Antigen-presenting glycoprotein CD1d, R3G1, CD1D, is the sole group-2 member of the CD1 family of major histocompatibility (MHC)-like glycoproteins. Expressed on the surface of various human antigen-presenting cells. They are related to the class I MHC molecules, and are involved in the presentation of lipid antigens to T cells. It is the only member of the group 2 CD1 molecules. CD1d presented lipid antigens activate a special class of T cells, known as Natural Killer T (NKT) cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines, typically represented by interferon-gamma and IL-4 production. Jayawardena-Wolf et al. described 2 different pathways of Cd1d trafficking to endosomal compartments in mouse cells. A tyrosine-based motif governs recycling between the plasma membrane and the endosome, while Cd1d associates, like major histocompatibility complex class II antigens, with the invariant chain, or II, in the endoplasmic reticulum. Both pathways enhance antigen presentation to Cd1d-restricted natural killer T cells.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Endoplasmic reticulum membrane; Single-pass type I membrane protein; Cell membrane; Basolateral cell membrane; Lysosome membrane; Endosome membrane
- Tissue details: Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.
- Research category: Angiogenesis,Cancer,Cardiovascular,Developmental Biology,Growth Factors,Growth Factors/Hormones,Nervous System Development,Organogenesis,Secreted,Signal Transduction,Signaling Pathways,Stem Cells,TGF Beta
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
