| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Host | |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Shipping | |
| Species | |
| Storage | |
| Target |
Immunotherapy, BiTEs
Product Overview
His-tagged Anti-CD20-Anti-CD3 IgM format bispecific antibody is a purified recombinant human bispecific antibody with T cell Engager. This bispecific antibody has been tested for specific activity in the functional reporter assay using NFAT-luc reporter Jurkat cell line (BPS Bioscience #60621) in the presence of CD20-CHO cells (BPS Bioscience #79624-H). The IgM Fc region is pentameric, so there are 5 copies of the anti-CD20 antibodies and J chain has anti-CD3 antibody.
| Clonality | Monoclonal (Bispecific) |
|---|---|
| Host | Human |
| Antibody Type | IgM |
| Isotype | IgM |
| Tag/Label | Anti-CD20: C-terminal His-Tag |
| Reactivity | Human |
| Purification | Ni-NTA affinity purification (HEK293) |
| Purity | ≥90% |
| Molecular Weight | Anti-CD20 HC: 66 kDa; Anti-CD20 LC: 26 kDa; Anti-CD3 VC: 45 kDa |
| Amino Acids | Anti-CD20 HC: 1-594(end); Anti-CD20 LC: 1-241(end); Anti-CD3 VC: 1-415(end) |
| Formulation | 8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol |
| Storage | Aqueous buffer solution. |
Validated Applications
- Western Blot
Assay conditions: Experimental design and assay protocol for measuring anti CD20-anti CD3 functional activity using NFAT-luc reporter Jurkat cell line: Jurkat effector cells with endogenous TCR/CD3 and transfected reporter NFAT-luc (BPS Bioscience #60621) are incubated with increasing concentrations of anti CD20 x anti CD3 bispecific antibody in the presence of CD20-CHO cells (BPS Bioscience #79624-H) or CHO cells (ATCC #CCL-61™).
1. Seed CHO and CD20-CHO cells at 30,000 cells/well and allow a few hours for the cells to attach in a 96-well clear bottom white plate.
2. Seed Jurkat cells at 30,000 cells/well.
3. Add the bispecific antibody at a recommended dilution range of 100 fM-100 nM. The bispecific antibody simultaneously binds to TCR/CD3 on the NFAT-luc Jurkat reporter cells and tumor antigen CD20 on CD20-CHO cells.
4. After 16 hours the luciferase activity is measured using ONE-Step™ luciferase assay (BPS Bioscience #60690) per recommended protocol. The bispecific antibody interaction stimulates NFAT-luciferase activity.
Immunogen / Antigen Information
Immunogen information not provided. Please refer to the product datasheet for detailed antigen information.
Cross-Reactivity & Specificity
Cross-reactivity data: Species reactivity as listed. No additional cross-reactivity data is available from the manufacturer.
Safety & Handling
Avoid freeze/thaw cycles.
For Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle with standard laboratory precautions (gloves, safety glasses, lab coat). Avoid freeze-thaw cycles. Consult SDS prior to use.
Related Products
- 60621
- 79624
- 100689
- 100836
This antibody has been validated for: Western Blot. Optimal dilutions and concentrations should be empirically determined for each specific application and sample type. Refer to the product datasheet for suggested starting conditions.
This antibody should be stored at the indicated temperature to maintain stability. Store at Aqueous buffer solution.. Avoid repeated freeze-thaw cycles, which can reduce antibody performance and increase aggregation. Centrifuge briefly after thawing and keep on ice during use.
Please refer to the BPS Bioscience datasheet for detailed immunogen information.
Cross-reactivity data is limited; refer to the product datasheet for available information.
BPS Bioscience offers multiple antibody formats for key checkpoint and immunotherapy targets. Contact BioHippo (support@biohippo.com) or click "Talk to a Scientist" to inquire about additional conjugate formats, bulk quantities, or custom formulations.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
