| Field | Specification |
|---|---|
| Target | |
| Alternative names | Macrophage scavenger receptor types I and II; Macrophage acetylated LDL receptor I and II; Scavenger receptor type A; SR-A; CD antigen CD204; Msr1; Scvr |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse CD204/Msr1 recombinant protein (Position: M1-D451). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects macrophage scavenger receptor types I and II (gene Msr1) in mouse samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 50.2 kDa, and the supplier reports an observed band at about 75–90 kDa.
Macrophage scavenger receptor 1 (MSR1, SR-A, CD204) is a class A scavenger receptor on macrophages. It binds and takes up modified low-density lipoproteins such as oxidized and acetylated LDL, as well as bacterial components and apoptotic cell debris, so it is studied in foam cell formation during atherosclerosis and in innate immunity.
| Target | Macrophage scavenger receptor types I and II (gene Msr1; UniProt P30204, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 50.2 kDa |
| Observed MW | 75–90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse RAW264.7 cells, mouse ANA-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): RAW264.7 cells.
Immunogen
Recombinant mouse CD204/Msr1 fragment (Met1–Asp451), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt places macrophage scavenger receptor types I and II in the membrane. Tissue expression noted by UniProt (mouse): Expressed in monocyte-derived macrophages. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
