Anti-Cd28 antibody [37.51] {AF647} (STJA0000270)

What it means: This is a Monoclonal antibody derived from a single B-cell clone that recognizes one specific epitope on the target antigen.

Why it matters:

• Monoclonal antibodies offer exceptional lot-to-lot reproducibility, critical for longitudinal studies and assay development.
• Preferred for flow cytometry, immunoprecipitation, and multiplex panels where cross-reactivity must be minimized.
• Single-epitope specificity makes them ideal for precisely defined quantitative assays.

How to interpret here: This Monoclonal antibody delivers consistent, highly specific performance and is well-suited for applications requiring stringent reproducibility.

Tip: Confirm the clone number matches your previous orders when re-ordering to ensure experimental continuity.

What it means: The host species is the animal in which this antibody was raised — in this case, Hamster.

Why it matters:

• Secondary antibodies and detection reagents must be matched to the primary antibody host (e.g., use anti-Hamster secondary for this Hamster primary antibody).
• Avoid using primary antibodies raised in the same species as the target tissue or cells to prevent non-specific background signal.

How to interpret here: Select a compatible anti-Hamster secondary antibody or detection system when using this antibody in your assay protocol.

What it means: This antibody has been confirmed or predicted to recognize the target antigen from the following species: Mouse.

Why it matters:

• Validating species reactivity ensures the antibody will detect the target in your specific experimental model organism.
• Cross-species reactivity is typically predicted from sequence homology and should be confirmed empirically for species not listed.

How to interpret here: This antibody has validated reactivity in Mouse samples. For unlisted species, consult the sequence homology data or contact the BioHippo team for guidance.

What it means: This antibody is conjugated with AF647, enabling direct detection without requiring a secondary antibody.

Why it matters:

• Direct-labeled antibodies simplify protocols, reduce assay time, and eliminate cross-reactivity issues associated with secondary antibodies.
• Particularly advantageous for multiplex assays, flow cytometry, and tissue sections where multiple antibodies from the same host species are used simultaneously.

How to interpret here: This AF647-conjugated antibody supports direct detection in your assay system — verify instrument or kit compatibility for the specific conjugate before ordering.

What it means: The antibody isotype is IgG, referring to the immunoglobulin class and its structural and effector properties.

Why it matters:

• Isotype determines which secondary detection antibodies are compatible — ensure your secondary specifically targets this isotype.
• In multiplex experiments, different IgG subclasses (IgG1, IgG2a, etc.) allow simultaneous detection of multiple targets without cross-interference.

How to interpret here: This antibody carries isotype IgG. Select a secondary antibody or detection system that specifically recognizes this isotype class for optimal signal-to-noise performance.

What it means: The clone number 37.51 uniquely identifies the specific B-cell hybridoma or recombinant construct from which this monoclonal antibody was derived.

Why it matters:

• Recording the clone number in your experimental notes ensures reproducibility and simplifies re-ordering the exact same antibody in future experiments.
• In publications, clone numbers allow independent labs to replicate your results with the same reagent.

How to interpret here: When re-ordering, verify that clone 37.51 matches the clone used in your previous experiments to maintain experimental consistency.

What it means: This antibody has been validated for use in the following research applications: Flow Cytometry.

Why it matters:

• Validated applications are confirmed by the manufacturer under defined assay conditions, providing confidence that the antibody performs as expected in these protocols.
• Using an antibody outside its validated application list may require additional optimization and is not guaranteed to produce reliable results.

How to interpret here: This antibody is tested and confirmed for Flow Cytometry. Refer to the product datasheet for recommended dilutions, controls, and protocol notes specific to each validated application.