| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Host | |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Species | |
| Storage | |
| Target |
Immunotherapy
Product Overview
Recombinant human antibody recognizing the CD3 binding region on immune cells.
| Clonality | Monoclonal |
|---|---|
| Host | Human |
| Antibody Type | IgG1 |
| Isotype | IgG1 |
| Reactivity | Human |
| Purification | Protein A affinity chromatography (HEK293) |
| Molecular Weight | Heavy Chain: 50 kDa, Light Chain: 23 kDa |
| Formulation | 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol. |
| Storage | Stable for at least 12 months at -80°C. Avoid freeze/thaw cycles. Store diluted antibody with carrier protein (BSA 0.1 - 0.5%) at 4°C, stable for 2 weeks. |
Validated Applications
- Western Blot
Assay conditions: IL-2 luciferase Jurkat reporter cells were incubated with anti-CD3 (BPS Cat. No 71274) in SF-RPMI for 24 hours. BPS luciferase reagent was added and luminescence was quantified. RLU = Relative Luminescence Unit normalized to wells with RPMI only.
Immunogen / Antigen Information
Immunogen information not provided. Please refer to the product datasheet for detailed antigen information.
Cross-Reactivity & Specificity
Cross-reactivity data: Species reactivity as listed. No additional cross-reactivity data is available from the manufacturer.
Safety & Handling
Avoid freeze/thaw cycles.
For Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle with standard laboratory precautions (gloves, safety glasses, lab coat). Avoid freeze-thaw cycles. Consult SDS prior to use.
Related Products
- 71274-1
- 71274-2
This antibody has been validated for: Western Blot. Optimal dilutions and concentrations should be empirically determined for each specific application and sample type. Refer to the product datasheet for suggested starting conditions.
This antibody should be stored at the indicated temperature to maintain stability. Store at Stable for at least 12 months at -80°C. Avoid freeze/thaw cycles. Store diluted antibody with carrier protein (BSA 0.1 - 0.5%) at 4°C, stable for 2 weeks.. Avoid repeated freeze-thaw cycles, which can reduce antibody performance and increase aggregation. Centrifuge briefly after thawing and keep on ice during use.
Please refer to the BPS Bioscience datasheet for detailed immunogen information.
Cross-reactivity data is limited; refer to the product datasheet for available information.
BPS Bioscience offers multiple antibody formats for key checkpoint and immunotherapy targets. Contact BioHippo (support@biohippo.com) or click "Talk to a Scientist" to inquire about additional conjugate formats, bulk quantities, or custom formulations.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
- Chatenoud, L., et al. PNAS. 1994; 91(1): 123-127.
- Herold, K., et al., NEJM. 2002; 346: 1692-1698.
