| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CD3 antigen, epsilon polypeptide (Predicted) ;Protein Cd3e ;Cd3e ;Cd3e_predicted ;rCG_58471 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Human thymocytes followed by Sezary T cells. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CD3 CD3E Antibody (Monoclonal, CA-3) is an antibody targeting CD3E. Common applications include IHC, ICC, Flow Cytometry. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: CA-3; isotype: Mouse IgG1; reactivity: Human; observed MW: 25 kDa; calculated MW: 21312 MW.
Boster Bio Anti-CD3 CD3E Antibody (Monoclonal, CA-3) catalog # MA1015. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CD3E — CD3e molecule, epsilon(CD3-TCR complex)
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: CA-3; Isotype: Mouse IgG1
- Species reactivity: Human
- Molecular weight guidance: Observed: 25 kDa; Calculated: 21312 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): CD3E: The CD3 complex mediates signal transduction.
Scientific background (datasheet): The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3 (formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.
Cellular localization (datasheet): CD3E: Membrane; Single-pass type I membraneprotein.|Cd3e: Cell membrane
Tissue details (datasheet): Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII. .
Sequence similarities (datasheet): Contains 1 Ig-like (immunoglobulin-like) domain.
Research relevance and current trends
- Commonly studied in contexts related to Adaptive Immunity,Hematopoietic Progenitors,Immunology,Stem Cells,T Cells.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
