| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | T-cell surface glycoprotein CD3 zeta chain;T-cell receptor T3 zeta chain;CD247;Cd247;Cd3z, Tcrz; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human CD3 zeta |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CD3 zeta CD247 Rabbit Monoclonal Antibody is an antibody targeting CD247. Common applications include WB, IHC, ICC, IF, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: IHI-3; isotype: IgG; reactivity: Human; observed MW: 65 kDa; calculated MW: 18637 MW.
Boster Bio Anti-CD3 zeta CD247 Rabbit Monoclonal Antibody catalog # M02421. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CD247 — T-cell surface glycoprotein CD3 zeta chain
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: IHI-3; Isotype: IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 65 kDa; Calculated: 18637 MW
Biological background
Protein function (datasheet): Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Cellular localization (datasheet): Membrane; Single-pass type I membrane protein.
Tissue details (datasheet): Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. .
Research relevance and current trends
- Commonly studied in contexts related to Adaptive Immunity,Hematopoietic Progenitors,Immunology,Stem Cells,T Cells.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
