| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD antigen CD33; CD33; SIGLEC3 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD33 Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADGE-3 is a rabbit monoclonal antibody that recognizes myeloid cell surface antigen CD33 in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 39.8 kDa, and the supplier reports an observed band at about 65–75 kDa.
CD33 (Siglec-3) is a sialic acid-binding immunoglobulin-like lectin expressed on myeloid cells and microglia. Through ITIM signaling it keeps immune cells in a resting state, and it is studied both as a target in acute myeloid leukemia and as a genetic risk factor in Alzheimer′s disease.
| Target | Myeloid cell surface antigen CD33 (gene CD33; UniProt P20138, human) |
|---|---|
| Clone | ADGE-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 39.8 kDa |
| Observed MW | 65–75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): THP-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CD33.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Monocytic/myeloid lineage cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
