| Field | Specification |
|---|---|
| Target | |
| Alternative names | Complement receptor type 1; C3b/C4b receptor; CD antigen CD35; CR1; C3BR |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD35 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25C32 is a rabbit monoclonal antibody that recognizes complement receptor type 1 (gene CR1) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 223.7 kDa, and the supplier reports an observed band at about 280 kDa.
Complement receptor 1 (CR1, CD35) is a membrane glycoprotein on erythrocytes, leukocytes and follicular dendritic cells that binds C3b and C4b. It helps clear immune complexes and opsonized particles and acts as a cofactor for inactivation of C3b and C4b, limiting complement activation.
| Target | Complement receptor type 1 (gene CR1; UniProt P17927, human) |
|---|---|
| Clone | 25C32 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 223.7 kDa |
| Observed MW | 280 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human tonsil cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CD35.
Reactivity Notes
The supplier lists reactivity with human. UniProt places complement receptor type 1 in the membrane. Tissue expression noted by UniProt (human): Present on erythrocytes, a subset of T cells, mature B cells, follicular dendritic cells, monocytes and granulocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
