| Field | Specification |
|---|---|
| Target | |
| Alternative names | ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1;3.2.2.6;2'-phospho-ADP-ribosyl cyclase;2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase;2.4.99.20;2'-phospho-cyclic-ADP-ribose transferase;ADP-ribosyl cyclase 1;ADPRC 1;Cyclic ADP-ribose hydrolase 1;cADPr hydrolase 1;T10;CD38;CD38; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD38 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GAD-3 is a rabbit monoclonal antibody that recognizes ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (gene CD38) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 34.3 kDa, and the supplier reports an observed band at about 45 kDa.
CD38 is a transmembrane enzyme that synthesizes and hydrolyzes cyclic ADP-ribose, a calcium-mobilizing second messenger, and is a major consumer of NAD+. It is highly expressed on plasma cells and activated lymphocytes, so it is used as an activation and plasma cell marker and studied in multiple myeloma and NAD+ metabolism.
| Target | ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (gene CD38; UniProt P28907, human) |
|---|---|
| Clone | GAD-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 34.3 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human Ramos cells, human Jurkat cells, human THP-1 cells, human Daudi cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human appendix tissue, human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human CD38.
Reactivity Notes
The supplier lists reactivity with human. UniProt places ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 in the cell surface and cell membrane. Tissue expression noted by UniProt (human): Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
