| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tumor necrosis factor receptor superfamily member 5;B-cell surface antigen CD40;Bp50;CD40L receptor;CDw40;CD40;CD40;TNFRSF5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human CD40/TNFRSF5 recombinant protein (Position: E21-R193). Human CD40/TNFRSF5 shares 60.2% amino acid (aa) sequence identity with mouse CD40/TNFRSF5. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CD40 (Tumor necrosis factor receptor superfamily member 5) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CD40 Antibody Picoband® catalog # PB10051. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human CD40/TNFRSF5 recombinant protein (Position: E21-R193). Human CD40/TNFRSF5 shares 60.2% amino acid (aa) sequence identity with mouse CD40/TNFRSF5. (reported region: E21-R193).
- Molecular weight context: reported MW: 43 kDa; calculated MW: 30619 MW
- Reactivity: Human
- Applications: ELISA, Flow Cytometry, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Tumor necrosis factor receptor superfamily member 5; Tumor necrosis factor receptor superfamily member 5. CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. It is a member of the tumor necrosis factor (TNF) receptor superfamily and is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin. Functional note: Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. Reported localization: Isoform I: Cell membrane; Single-pass type I membrane protein. Expression/tissue context: B-cells and in primary carcinomas.
Research relevance and current trends
- Adaptive Immunity: Researchers commonly examine how CD40 (Tumor necrosis factor receptor superfamily member 5) relates to this theme using model systems and orthogonal readouts.
- Atherosclerosis: Researchers commonly examine how CD40 (Tumor necrosis factor receptor superfamily member 5) relates to this theme using model systems and orthogonal readouts.
- B Cells: Researchers commonly examine how CD40 (Tumor necrosis factor receptor superfamily member 5) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CD40 (Tumor necrosis factor receptor superfamily member 5) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CD40 (Tumor necrosis factor receptor superfamily member 5) across tissue regions and cell types using matched controls.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
