| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor receptor superfamily member 5;B-cell surface antigen CD40;Bp50;CD40L receptor;CD40;Cd40;Tnfrsf5; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse CD40/TNFRSF5 recombinant protein (Position: L20-R193). Mouse CD40/TNFRSF5 shares 60.7% amino acid (aa) sequence identity with human CD40/TNFRSF5. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes tumor necrosis factor receptor superfamily member 5 (gene Cd40) in human and mouse samples and is validated for ELISA, flow cytometry, IHC, ICC/IF and Western blot. The predicted molecular weight is 32.1 kDa, and the supplier reports an observed band at about 40 kDa.
CD40 is a TNF receptor superfamily member expressed on B cells, dendritic cells, macrophages and other cells. Binding of CD40 ligand from activated T cells drives B-cell proliferation, class switching and germinal center formation, and activates antigen-presenting cells, making CD40 central to adaptive immunity research.
| Target | Tumor necrosis factor receptor superfamily member 5 (gene Cd40; UniProt P27512, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 32.1 kDa |
| Observed MW | 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL |
|---|---|
| IHC (frozen sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
| Western blot | 0.1–0.5 µg/mL |
| Flow cytometry | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse lymphaden tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): U937 cells.
Immunogen
Recombinant mouse CD40/TNFRSF5 fragment (Leu20–Arg193), expressed in E. coli. Sequence identity with the human ortholog: 60.7%.
Reactivity Notes
The supplier lists reactivity with human and mouse. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yidi Qu et al. (2023) The involvement of Th1 cell differentiation in the anti-tumor effect of purified polysaccharide from Sanghuangporus vaninii in colorectal cancer via multi-omics analysis. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2023.123927
Hong-Xue Meng et al. (2015) Decreased expression of follicular dendritic cell–secreted protein correlates with increased immunoglobulin A production in the tonsils of individuals with immunoglobulin A nephropathy. Translational Research. 10.1016/j.trsl.2015.04.004
Zhan Gao et al. (2024) Dendritic cell-based biomimetic nanoparticles for foot-and-mouth disease induce robust cellular immunity. Antiviral Research. 10.1016/j.antiviral.2024.106011
Du Li-Cheng et al. (2014) VEGF-D-induced draining lymphatic enlargement and tumor lymphangiogenesis promote lymph node metastasis in a xenograft model of ovarian carcinoma. Reproductive Biology and Endocrinology. 10.1186/1477-7827-12-14
Ping Chen et al. (2009) Coxsackievirus B3 infection promotes generation of myeloid dendritic cells from bone marrow and accumulation in the myocardium. International Immunopharmacology. 10.1016/j.intimp.2009.07.014
