| Field | Specification |
|---|---|
| Target | |
| Alternative names | Receptor-type tyrosine-protein phosphatase C;3.1.3.48;Leukocyte common antigen;L-CA;T200;CD45;PTPRC;CD45; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD45 recombinant protein (Position: R1113-S1304). Human CD45 shares 68% amino acid (aa) sequence identity with both mouse and rat CD45. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects receptor-type tyrosine-protein phosphatase C (gene PTPRC) in human samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 147.5 kDa, and the supplier reports an observed band at about 180–250 kDa.
CD45 (PTPRC) is a transmembrane tyrosine phosphatase present on all nucleated blood cells. By removing inhibitory phosphates from Src family kinases such as LCK and FYN it enables T- and B-cell antigen receptor signaling, and its isoforms (for example CD45RA and CD45RO) are widely used to mark leukocyte subsets.
| Target | Receptor-type tyrosine-protein phosphatase C (gene PTPRC; UniProt P08575, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 147.5 kDa |
| Observed MW | 180–250 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human Jurkat cells, human Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human tonsil tissue.
Samples with a confirmed signal (flow cytometry): THP-1 cells.
Immunogen
Recombinant human CD45 fragment (Arg1113–Ser1304), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 68%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places receptor-type tyrosine-protein phosphatase C in the cell membrane, membrane raft and synapse. Tissue expression noted by UniProt (human): Isoform 1: Detected in thymocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhou Xihong et al. (2018) Long-Term l-Serine Administration Reduces Food Intake and Improves Oxidative Stress and Sirt1/NFκB Signaling in the Hypothalamus of Aging Mice. Frontiers in Endocrinology. 10.3389/fendo.2018.00476
