| Field | Specification |
|---|---|
| Target | |
| Alternative names | Receptor-type tyrosine-protein phosphatase C;3.1.3.48;Leukocyte common antigen;L-CA;T200;CD45;PTPRC;CD45; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD45 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CBI-16 is a rabbit monoclonal antibody that recognizes receptor-type tyrosine-protein phosphatase C (gene PTPRC) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 147.5 kDa, and the supplier reports an observed band at about 200 kDa.
CD45 (PTPRC) is a transmembrane tyrosine phosphatase present on all nucleated blood cells. By removing inhibitory phosphates from Src family kinases such as LCK and FYN it enables T- and B-cell antigen receptor signaling, and its isoforms (for example CD45RA and CD45RO) are widely used to mark leukocyte subsets.
| Target | Receptor-type tyrosine-protein phosphatase C (gene PTPRC; UniProt P08575, human) |
|---|---|
| Clone | CBI-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 147.5 kDa |
| Observed MW | 200 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human CD45.
Reactivity Notes
The supplier lists reactivity with human. UniProt places receptor-type tyrosine-protein phosphatase C in the cell membrane, membrane raft and synapse. Tissue expression noted by UniProt (human): Isoform 1: Detected in thymocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
