| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | AHUS2 antibody; Antigen defined by monoclonal antibody; TRA 2 10 antibody; Antigen identified by monoclonal antibody; TRA 2 10 antibody; CD46 antibody; CD46 antigen antibody; CD46 antigen complement regulatory protein antibody; CD46 molecule antibody; CD46 molecule complement regulatory protein antibody; Complement membrane cofactor protein antibody; MCP antibody; MCP_HUMAN antibody; Measles virus receptor antibody; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) antibody; Membrane cofactor protein antibody; MGC26544 antibody;MIC10 antibody; TLX antibody; TRA2.10 antibody; Trophoblast leucocyte common antigen antibody; Trophoblast leukocyte common antigen antibody; Trophoblast lymphocyte cross reactive antigen antibody |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CD46. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CD46 Antibody Picoband® (monoclonal, 9E9) is an antibody for CD46 detection raised in Mouse (Monoclonal, clone Clone: 9E9, Mouse IgG1), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: CD46 (CD46 molecule, complement regulatory protein); UniProt: P15529
- Antibody format: Mouse, Monoclonal, clone Clone: 9E9, Mouse IgG1
- Molecular weight: 50-70 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-CD46 Antibody Picoband® (monoclonal, 9E9) catalog # M00377-2.
Biological background
Biological context: Acts as a cofactor for complement factor I,a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10,and therefore are thought to prevent autoimmunity.
Expression and localization notes: cellular localization: Secreted., tissue context: Expressed by all cells except erythrocytes..
Common research applications
- Western blotting (WB): Compare CD46 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of CD46 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify CD46-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: CD46 complement regulatory protein also known as CD46 (cluster of differentiation 46) and Membrane Cofactor Protein is a protein which in humans is encoded by the CD46 gene. The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. And the encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Secreted.
- Tissue details: Expressed by all cells except erythrocytes.
- Research category: Cell Biology,Cell Cycle,Immunology,Innate Immunity,Kinases/Phosphatases,Lipid Signaling,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction,Signaling Pathway,TLR Signaling
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
