| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; CD5; CD5; LEU1 |
| Cellular Localization | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived human CD5 recombinant protein (Position: R25-L495). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CD5 Antibody Picoband® (monoclonal, 4E2) is an antibody for CD5 detection raised in Mouse (Monoclonal, clone Clone: 4E2, Mouse IgG1), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: CD5 (CD5 molecule); UniProt: P06127
- Antibody format: Mouse, Monoclonal, clone Clone: 4E2, Mouse IgG1
- Molecular weight: 67 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-CD5 Antibody Picoband® (monoclonal, 4E2) catalog # M00480-2.
Biological background
Biological context: May act as a receptor in regulating T-cell proliferation.
Expression and localization notes: cellular localization: Cell membrane; Single-pass type I membrane protein, tissue context: Brain, liver, placenta, lymphocytes and erythrocytes..
Common research applications
- Western blotting (WB): Compare CD5 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of CD5 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify CD5-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: CD5 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. In humans, the gene is located on the long arm of chromosome 11. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane; Single-pass type I membrane protein
- Tissue details: Brain, liver, placenta, lymphocytes and erythrocytes.
- Research category: Cell Adhesion,Cell Type Markers,Cytoskeleton/ECM,Hematopoietic Progenitors,Immunology,Integrins,Myeloid,Signal Transduction,Stem Cells
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
