| Field | Specification |
|---|---|
| Target | |
| Alternative names | CAMPATH-1 antigen; Lymphocyte differentiation antigen B7; CD antigen CD52; Cd52; Cdw52; Mb7 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD52 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEIF-3 is a rabbit monoclonal antibody that recognizes CAMPATH-1 antigen (gene Cd52) in mouse samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 7.8 kDa, and the supplier reports an observed band at about 15–20 kDa.
CD52 (CAMPATH-1 antigen) is a small GPI-anchored glycoprotein expressed on mature lymphocytes, monocytes and cells of the male reproductive tract. Its biological function is not fully understood, but its abundance on lymphocytes has made it a well-known target for lymphocyte depletion in research and therapy.
| Target | CAMPATH-1 antigen (gene Cd52; UniProt Q64389, mouse) |
|---|---|
| Clone | AEIF-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 7.8 kDa |
| Observed MW | 15–20 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): mouse spleen cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CD52.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt places CAMPATH-1 antigen in the cell membrane. Tissue expression noted by UniProt (mouse): Expressed on lymphohematopoietic tissues, including thymus, spleen, and bone marrow, but not in liver, kidney, and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
