| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Histone deacetylase 11;HD11;3.5.1.98;HDAC11; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human CD53 May be involved in growth regulation in hematopoietic cells. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-CD53 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AFFG-3; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-CD53 Monoclonal Antibody catalog # M06249. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CD53 (Histone deacetylase 11).
- Antibody format: Monoclonal; clone AFFG-3; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
CD53 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. . Reported cellular localization context: Nucleus. Predominantly nuclear. Tissue expression notes (as provided): Weakly expressed in most tissues. Strongly expressed in brain, heart, skeletal muscle, kidney and testis.
Research relevance and current trends
- Research context keywords from the source record include: Adapters,Cell Type Markers,Immunology,Signal Transduction,Transmembrane.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate CD53 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CD53 expression by Western blot in cell or tissue lysates, Detect CD53 in FFPE tissue sections by immunohistochemistry, Localize CD53 by immunofluorescence/immunocytochemistry in cultured cells, Quantify CD53-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 60 kDa; calculated MW: 39183 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 60 kDa
- Cellular localization (provided): Nucleus. Predominantly nuclear.
- Tissue details (provided): Weakly expressed in most tissues. Strongly expressed in brain, heart, skeletal muscle, kidney and testis.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
