| Field | Specification |
|---|---|
| Target | |
| Alternative names | CD59A glycoprotein; MAC-inhibitory protein; MAC-IP; Membrane attack complex inhibition factor; MACIF; Protectin; CD59; Cd59a; Cd59 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse CD59 recombinant protein (Position: L24-S96). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets CD59A glycoprotein in mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 13.6 kDa, and the supplier reports an observed band at about 20 kDa.
In mouse, CD59a is the main, broadly expressed form of CD59, the GPI-anchored inhibitor of the complement membrane attack complex. By binding C8 and C9 it prevents pore formation and protects host cells from complement-mediated lysis, and CD59a-deficient mice are used to study complement-driven tissue injury.
| Target | CD59A glycoprotein (gene Cd59a; UniProt O55186, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 13.6 kDa |
| Observed MW | 20 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat liver tissue, rat kidney tissue, rat spleen tissue, rat brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue, mouse thymus tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant mouse CD59 fragment (Leu24–Ser96), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes CD59A glycoprotein as secreted and also located in the cell membrane. Tissue expression noted by UniProt (mouse): Expressed in all tissues examined (liver, kidney, spleen, thymus, brain and heart). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
