| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CD59 glycoprotein;1F5 antigen;20 kDa homologous restriction factor;HRF-20;HRF20;MAC-inhibitory protein;MAC-IP;MEM43 antigen;Membrane attack complex inhibition factor;MACIF;Membrane inhibitor of reactive lysis;MIRL;Protectin;CD59;CD59;MIC11, MIN1, MIN2, MIN3, MSK21; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human CD59 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CD59 Rabbit Monoclonal Antibody is an antibody targeting CD59. Common applications include WB, ICC, IF, IP, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AABE-3; isotype: Rabbit IgG; reactivity: Human; observed MW: 120 kDa; calculated MW: 14177 MW.
Boster Bio Anti-CD59 Rabbit Monoclonal Antibody catalog # M00914-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CD59 — CD59 glycoprotein
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AABE-3; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 120 kDa; Calculated: 14177 MW
Biological background
Protein function (datasheet): Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
Cellular localization (datasheet): Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Soluble form found in a number of tissues.
Tissue details (datasheet): Detected in caudate, nucleus accumbens and in the olfactory tubercle. .
Research relevance and current trends
- Commonly studied in contexts related to Atherosclerosis,Cardiovascular,Cell Type Markers,Complement,Hematopoietic Progenitors,Immunology,Innate Immunity,Protein Phosphorylation,Signal Transduction,Stem Cells,Surface Molecules,Tyrosine Kinases,Vascular Inflammation.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
