| Field | Specification |
|---|---|
| Target | |
| Alternative names | CD59 glycoprotein;1F5 antigen;20 kDa homologous restriction factor;HRF-20;HRF20;MAC-inhibitory protein;MAC-IP;MEM43 antigen;Membrane attack complex inhibition factor;MACIF;Membrane inhibitor of reactive lysis;MIRL;Protectin;CD59;CD59;MIC11, MIN1, MIN2, MIN3, MSK21; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD59 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AABE-3 is a rabbit monoclonal antibody that recognizes CD59 glycoprotein in human samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 14.2 kDa, and the supplier reports an observed band at about 18 kDa.
CD59 (protectin) is a GPI-anchored protein that blocks formation of the complement membrane attack complex by binding C8 and C9 and preventing C9 polymerization. It protects host cells from complement-mediated lysis, and its loss on blood cells is a hallmark of paroxysmal nocturnal hemoglobinuria.
| Target | CD59 glycoprotein (gene CD59; UniProt P13987, human) |
|---|---|
| Clone | AABE-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 14.2 kDa |
| Observed MW | 18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human U-87MG cells, human A549 cells, human SIHA cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): BxPC-3 cells.
Immunogen
Synthetic peptide based on the sequence of human CD59.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes CD59 glycoprotein as secreted and also located in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Linzhu et al. (2026) CD59 silencing enhances doxorubicin sensitivity in DLBCL cells and is associated with poor prognosis in patients treated with CHOP regimen. Clinical and Experimental Medicine. 10.1007/s10238-026-02134-2
