| Field | Specification |
|---|---|
| Target | |
| Alternative names | P-selectin;CD62 antigen-like family member P;Granule membrane protein 140;GMP-140;Leukocyte-endothelial cell adhesion molecule 3;LECAM3;Platelet activation dependent granule-external membrane protein;PADGEM;CD62P;SELP;GMRP, GRMP; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CD62P recombinant protein (Position: W42-G271). Human CD62P shares 75.2% and 75.7% amino acid (aa) sequence identity with mouse and rat CD62P, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes P-selectin (gene SELP) in human samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 90.8 kDa, and the supplier reports an observed band at about 140 kDa.
P-selectin (SELP, CD62P) is an adhesion molecule stored in platelet α-granules and endothelial Weibel-Palade bodies and moved to the cell surface upon activation. It binds PSGL-1 on leukocytes to start their rolling during inflammation and links platelets with leukocytes, and it is widely used as a platelet activation marker.
| Target | P-selectin (gene SELP; UniProt P16109, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 90.8 kDa |
| Observed MW | 140 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human tonsil tissue, human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human CD62P fragment (Trp42–Gly271), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 75.2% and 75.7%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places P-selectin in the cell membrane. Tissue expression noted by UniProt (human): Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhenjiang Zheng et al. (2024) Growth inhibition of pancreatic cancer by targeted delivery of gemcitabine via fucoidan-coated pH-sensitive liposomes. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2024.134517
