| Field | Specification |
|---|---|
| Target | |
| Alternative names | CD70 antigen; CD27 ligand; CD27-L; Tumor necrosis factor ligand superfamily member 7; CD antigen CD70; Cd70; Cd27l; Cd27lg; Tnfsf7 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Cd70 recombinant protein (Position: C36-Q191). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets CD70 antigen in mouse and rat samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 21.9 kDa, and the supplier reports an observed band at about 30 kDa.
CD70 is a TNF family ligand expressed transiently on activated lymphocytes and dendritic cells. It binds the receptor CD27 to support T-cell activation, expansion and memory formation, and it is studied in immune co-stimulation and in tumors that express it aberrantly.
| Target | CD70 antigen (gene Cd70; UniProt O55237, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 21.9 kDa |
| Observed MW | 30 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat spleen tissue, rat thymus tissue, rat lung tissue, rat L6 cells, rat PC-12 cells, mouse B16 cells, mouse spleen tissue, mouse thymus tissue, mouse lung tissue, mouse RAW264.7 cells, mouse ANA-1 cells, mouse SP2/0 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse lymphonode tissue, mouse spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse Cd70 fragment (Cys36–Gln191), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places CD70 antigen in the cell membrane. Tissue expression noted by UniProt (mouse): Very low level of expression. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Gao Liyun et al. (2025) Identification of m6A methyltransferase-related WTAP and ZC3H13 predicts immune infiltrates in glioblastoma. Scientific Reports. 10.1038/s41598-025-88671-4
