| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; Ig-beta; Immunoglobulin-associated B29 protein; CD79b; CD79B; B29, IGB |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CD79b recombinant protein (Position: A29-E229). Human CD79b shares 70% amino acid (aa) sequence identity with mouse CD79b. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CD79b Antibody Picoband®(monoclonal, 6H11) is an antibody for CD79B detection raised in Mouse (Monoclonal, clone Clone: 6H11, Mouse IgG1), with reported reactivity: Human,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: CD79B (B-cell antigen receptor complex-associated protein beta chain); UniProt: P40259
- Antibody format: Mouse, Monoclonal, clone Clone: 6H11, Mouse IgG1
- Molecular weight: 35 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-CD79b Antibody Picoband® (monoclonal, 6H11) catalog # M01399-1.
Biological background
Biological context: Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Expression and localization notes: cellular localization: Nucleolus. Perinuclear region., tissue context: Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma..
Common research applications
- Western blotting (WB): Compare CD79B levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of CD79B in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify CD79B-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: CD79b molecule, immunoglobulin-associated beta, also known as CD79B (Cluster of Differentiation 79B), is a human gene. By fluorescence in situ hybridization, It is mapped to 17q23.3. The CD79B protein together with the related CD79A protein, forms a dimer associated with membrane bound immunoglobulin in B-cells, thus forming the B-cell antigen receptor (BCR) which is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). CD79b also can enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleolus. Perinuclear region.
- Tissue details: Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
- Research category: DNA/RNA,DNA Damage & Repair,DNA Damage Response,DNA Synthesis,Epigenetics and Nuclear Signaling
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
