| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | T-cell surface glycoprotein CD8 beta chain;CD8 antigen 37 kDa chain;OX-8 membrane antigen;CD8b;Cd8b;Cd8b1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Human thymocytes followed by peripheral blood T cells. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CD8 Cd8A Antibody (Monoclonal, CA-8) is an antibody targeting CD8A; CD8B. Common applications include IHC, ICC, Flow Cytometry. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: CA-8; isotype: Mouse IgG2a; reactivity: Human; observed MW: 25 kDa; calculated MW: 23400 MW.
Boster Bio Anti-CD8 Cd8A Antibody (Monoclonal, CA-8) catalog # MA1017. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CD8A; CD8B
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: CA-8; Isotype: Mouse IgG2a
- Species reactivity: Human
- Molecular weight guidance: Observed: 25 kDa; Calculated: 23400 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
Scientific background (datasheet): The human lymphocyte differentiation antigen CD8 is encoded by a single gene that gives rise to a 33- to 34-kDa glycoprotein expressed on the cell surface as a dimer and in higher molecular mass forms.
Cellular localization (datasheet): Membrane; Single-pass type I membrane protein.
Tissue details (datasheet): Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII. .
Sequence similarities (datasheet): Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Research relevance and current trends
- Commonly studied in contexts related to Adaptive Immunity,Antiviral Signaling,Hematopoietic Progenitors,Immune System Diseases,Immunology,Lymphoid,Myeloid,Stem Cells,T Cells,T Lymphocytic Lineage.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
