| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-cell surface glycoprotein CD8 beta chain;CD8 antigen 37 kDa chain;OX-8 membrane antigen;CD8b;Cd8b;Cd8b1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Human thymocytes followed by peripheral blood T cells. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CA-8 is a mouse monoclonal antibody that recognizes T-cell surface glycoprotein CD8 alpha chain (gene CD8A) in human samples and is validated for IHC, ICC/IF and flow cytometry.
CD8α (CD8A) is a transmembrane glycoprotein that forms the CD8 co-receptor, usually as a CD8αβ heterodimer on cytotoxic T cells and as a CD8αα homodimer on some other lymphocytes. It binds MHC class I and recruits LCK to enhance T-cell receptor signaling, and it is the standard marker for cytotoxic T cells.
| Target | T-cell surface glycoprotein CD8 alpha chain (gene CD8A; UniProt P01732, human) |
|---|---|
| Clone | CA-8 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2a |
| Reactivity | Human |
| Purification | Ascites |
| Form | Lyophilized |
| Formulation | 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers |
| Reconstitution | 100 µg/mL after adding 1 mL PBS |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (frozen sections) | 1 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
| Flow cytometry | 1–3 µg/1×106 cells (Human) |
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Human thymocytes followed by peripheral blood T cells.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): CD8 on thymus-derived T-cells usually consists of a disulfide-linked alpha/CD8A and a beta/CD8B chain.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhuo-Ran Wang et al. (2024) Establishing prognostic models for intrahepatic cholangiocarcinoma based on immune cells. World Journal of Gastrointestinal Oncology. 10.4251/wjgo.v16.i10.4092
