Anti-CD82 Antibody

Overview

Anti-CD82 Antibody is an antibody for Cd82 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: Cd82 (Tumor necrosis factor receptor superfamily member 14); UniProt: P40237
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 80-85 kDa, calculated 30392 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-CD82 Antibody Picoband® catalog # A02300-1.

Biological background

Biological context: Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells. .

Expression and localization notes: cellular localization: Membrane ; Single-pass type I membrane protein ., tissue context: Widely expressed, with the highest expression in lung, spleen and thymus..

Common research applications

• Western blotting (WB): Compare Cd82 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of Cd82 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify Cd82-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: CD82 (Cluster of Differentiation 82), also named KAI1, is a human protein encoded by the CD82 gene. The gene is mapped to 11p11.2. This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. The expression of CD82 protein appears to be correlated with lymph node metastasis in esophageal squamous cell carcinoma (ESCC). And the CD82 overexpression can suppress tumor invasiveness and metastatic potential by inducing MMP9 inactivation via upregulation of TIMP1.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Membrane ; Single-pass type I membrane protein .
• Tissue details: Widely expressed, with the highest expression in lung, spleen and thymus.
• Research category: Antiviral Signaling,Cancer,Cytokines,Growth Factors,Growth Factors/Hormones,Immune System Diseases,Immunology,Innate Immunity,Signal Transduction,TNF Superfamily

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.