| Field | Specification |
|---|---|
| Target | |
| Alternative names | CD9 antigen;CD9;Cd9; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse CD9 recombinant protein (Position: T110-I193). Mouse CD9 shares 77.4% and 86.9% amino acid (aa) sequence identity with human and rat CD9, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets CD9 antigen in mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 25.3 kDa, and the supplier reports an observed band at about 25 kDa.
CD9 is a tetraspanin that associates with integrins and other membrane proteins to influence cell adhesion, migration and platelet activation. It is required for sperm–egg fusion on the oocyte surface, and it is one of the most commonly used exosome markers.
| Target | CD9 antigen (gene Cd9; UniProt P40240, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 25.3 kDa |
| Observed MW | 25 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Mouse) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
Samples with a confirmed band (WB): mouse kidney tissue, rat thymus tissue, rat lung tissue, mouse spleen tissue, mouse lung tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HEPA1–6 cells.
Samples with a confirmed signal (flow cytometry): RAW264.7 cells.
Immunogen
Recombinant mouse CD9 fragment (Thr110–Ile193), expressed in E. coli. Sequence identity with the human and rat orthologs: 77.4% and 86.9%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes CD9 antigen as secreted and also located in the cell membrane and membrane. Tissue expression noted by UniProt (mouse): Expressed predominantly in the peripheral nervous system. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhen Chen et al. (2024) Engineering Macrophage-Derived Exosome to Deliver Pirfenidone: A Novel Approach to Combat Silicotic Pulmonary Fibrosis. Advanced Healthcare Materials. 10.1002/adhm.202403227
Taoping Zhang et al. (2025) IL-16 exerts anti-rabies virus effects through CD9 on the surface of viral particles. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.141042
Kaili Wang et al. (2026) Exosome-mediated cross-species miRNA regulation: Bovine Milk-derived miR-320a attenuates ovalbumin-induced food allergy by suppressing STAT3. Food Research International. 10.1016/j.foodres.2026.118466
Qiling Yuan et al. (2024) M2 Macrophage-Derived Extracellular Vesicles Encapsulated in Hyaluronic Acid Alleviate Osteoarthritis by Modulating Macrophage Polarization. ACS Biomaterials Science & Engineering. 10.1021/acsbiomaterials.3c01833
Cao Guijun et al. (2020) Exosomes derived from circRNA Rtn4-modified BMSCs attenuate TNF-α-induced cytotoxicity and apoptosis in murine MC3T3-E1 cells by sponging miR-146a. Bioscience Reports. 10.1042/BSR20193436
