| Field | Specification |
|---|---|
| Target | |
| Alternative names | Thy-1 membrane glycoprotein;Thy-1 antigen;CD90;Thy1;Thy-1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse CD90/Thy1 recombinant protein (Position: Q20-C131). Mouse CD90/Thy1 shares 63.4% and 81.3% amino acid (aa) sequence identity with human and rat CD90/Thy1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects thy-1 membrane glycoprotein (gene Thy1) in mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 18.1 kDa, and the supplier reports an observed band at about 22 kDa.
Thy-1 (THY1, CD90) is a small GPI-anchored glycoprotein of the immunoglobulin superfamily found on the surface of neurons, thymocytes, T cells in mice, fibroblasts and some stem cells. It takes part in cell–cell and cell–matrix interactions, including in the nervous system. CD90 is widely used as a marker for T cells in mice, mesenchymal stromal cells and hematopoietic stem cells.
| Target | Thy-1 membrane glycoprotein (gene Thy1; UniProt P01831, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 18.1 kDa |
| Observed MW | 22 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Rat) |
Samples with a confirmed band (WB): rat brain tissue, rai thymus tissue, mouse brain tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse spleen tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): rat brain tissue.
Immunogen
Recombinant mouse CD90/Thy1 fragment (Gln20–Cys131), expressed in E. coli. Sequence identity with the human and rat orthologs: 63.4% and 81.3%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places thy-1 membrane glycoprotein in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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