| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Thy-1 membrane glycoprotein;Thy-1 antigen;CD90;Thy1;Thy-1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived mouse CD90/Thy1 recombinant protein (Position: Q20-C131). Mouse CD90/Thy1 shares 63.4% and 81.3% amino acid (aa) sequence identity with human and rat CD90/Thy1, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Thy1 (Thy-1 membrane glycoprotein) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CD90/Thy1 Antibody Picoband® catalog # A01818. Tested in IF, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived mouse CD90/Thy1 recombinant protein (Position: Q20-C131). Mouse CD90/Thy1 shares 63.4% and 81.3% amino acid (aa) sequence identity with human and rat CD90/Thy1, respectively. (reported region: Q20-C131).
- Molecular weight context: reported MW: 22 kDa; calculated MW: 18080 MW
- Reactivity: Mouse,Rat
- Applications: IF, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Thy-1 membrane glycoprotein; Thy-1 membrane glycoprotein. CD90 (Cluster of Differentiation 90) or Thy-1 is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. The CD90 gene is mapped to 11q23.3. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Functional note: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. Reported localization: Cell membrane; Lipid-anchor, GPI-anchor. Expression/tissue context: Detected adjacent to nodes of Ranvier in juxtaparanodal zones in spinal cord nerve fibers, but also in paranodal regions in some myelinated spinal cord axons (at protein level) (PubMed:11086297). Detected in the islet of Langerhans (PubMed:21483673).
Research relevance and current trends
- Neuroscience: Researchers commonly examine how Thy1 (Thy-1 membrane glycoprotein) relates to this theme using model systems and orthogonal readouts.
- Stem Cells: Researchers commonly examine how Thy1 (Thy-1 membrane glycoprotein) relates to this theme using model systems and orthogonal readouts.
- Synapse Marker: Researchers commonly examine how Thy1 (Thy-1 membrane glycoprotein) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Thy1 (Thy-1 membrane glycoprotein) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of Thy1 (Thy-1 membrane glycoprotein) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
