| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Meiotic recombination protein REC8 homolog; Cohesin Rec8p; REC8; REC8L1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CD93, which shares 90.9% amino acid (aa) sequence identity with mouse and rat CD93. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CD93 Antibody is an antibody for CD93 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: CD93 (REC8 meiotic recombination protein); UniProt: Q9NPY3
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 150 kDa, calculated 56903 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-CD93 Antibody Picoband® catalog # A04939-1.
Biological background
Biological context: Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.
Expression and localization notes: cellular localization: Nucleus. Chromosome. Centromere., tissue context: Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa..
Common research applications
- Western blotting (WB): Compare CD93 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of CD93 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify CD93-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: CD93 (Cluster of Differentiation 93) is a protein that in humans is encoded by the CD93 gene. The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus. Chromosome. Centromere.
- Tissue details: Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa.
- Research category: Vasculature
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
