| Field | Specification |
|---|---|
| Target | |
| Alternative names | Translation initiation factor eIF2 assembly protein; Cell division cycle protein 123 homolog; Protein D123; HT-1080; PZ32; CDC123; C10orf7; D123 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CDC123 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24C82 is a rabbit monoclonal antibody that recognizes translation initiation factor eIF2 assembly protein (gene CDC123) in human, mouse and rat samples and is validated for Western blot, immunoprecipitation and flow cytometry. The predicted molecular weight is 39.1 kDa, and the supplier reports an observed band at about 44 kDa.
CDC123 is a cytoplasmic protein required for assembly of eukaryotic initiation factor 2 (eIF2). It binds the eIF2γ subunit in an ATP-dependent manner and helps it join with the α and β subunits, supporting translation initiation and cell proliferation.
| Target | Translation initiation factor eIF2 assembly protein (gene CDC123; UniProt O75794, human) |
|---|---|
| Clone | 24C82 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 39.1 kDa |
| Observed MW | 44 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CDC123.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places translation initiation factor eIF2 assembly protein in the cytoplasm. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
