| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein Cdc25c ;Cdc25c ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Recombinant human Cdc25c. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CDC25C Antibody (Monoclonal, DCS-193) is an antibody targeting CDC25C. Common applications include WB. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: DCS-193; isotype: Mouse IgG1; reactivity: Human; observed MW: 25 kDa; calculated MW: 50896 MW.
Boster Bio Anti-CDC25C Antibody (Monoclonal, DCS-193) catalog # MA1018. Tested in WB applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CDC25C — cell division cycle 25 homolog C(S. pombe)
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: DCS-193; Isotype: Mouse IgG1
- Species reactivity: Human
- Molecular weight guidance: Observed: 25 kDa; Calculated: 50896 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): CDC25C: Functions as a dosage-dependent inducer in mitoticcontrol. Tyrosine protein phosphatase required for progression ofthe cell cycle. When phosphorylated, highly effective inactivating G2 cells into prophase. ly dephosphorylates CDK1and activates its kinase activity.
Scientific background (datasheet): Cell division sycle (CDC25) gene product is a protein-tyrosine phosphatase, activates a partially purified p34 (cdc2)/cyclin B complex. The cdc25 protein also shares homology with a protein phosphatase with activity against both tyrosine and serine (and thus probably threonine) phosphate residues. CDC25C is expressed predominantly in G2 phase in healer cells. The human gene encodes a protein with a predicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37% sequence identity with the fission yeast cdc25 mitotic inducer. CDC25C gene is mapped to 5q31.
Cellular localization (datasheet): CDC25C: Nucleus |Cdc25c: Nucleus.
Sequence similarities (datasheet): Belongs to the MPI phosphatase family.
Research relevance and current trends
- Commonly studied in contexts related to Cell Biology,Cell Cycle,Epigenetics and Nuclear Signaling,Kinases/Phosphatases.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
