| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | M-phase inducer phosphatase 3;3.1.3.48;Dual specificity phosphatase Cdc25C;CDC25C; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Cdc25C recombinant protein (Position: K175-P473). Human Cdc25C shares 78.2% amino acid (aa) sequence identity with mouse Cdc25C. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CDC25C (M-phase inducer phosphatase 3) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Cdc25C Antibody Picoband® catalog # PB9756. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human Cdc25C recombinant protein (Position: K175-P473). Human Cdc25C shares 78.2% amino acid (aa) sequence identity with mouse Cdc25C. (reported region: K175-P473).
- Molecular weight context: reported MW: 53 kDa; calculated MW: 53 kDa
- Reactivity: Human,Mouse,Rat
- Applications: WB, ICC/IF, Flow Cytometry
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
M-phase inducer phosphatase 3; M-phase inducer phosphatase 3. M-phase inducer phosphatase 3 is an enzyme that in humans is encoded by the CDC25C gene. This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It s dephosphorylation of cyclin B-bound CDC2 (CDK1) and triggers entry into mitosis. Also, it is thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. Functional note: Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. ly dephosphorylates CDK1 and activates its kinase activity. . Reported localization: Nucleus . Expression/tissue context: Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart. .
Research relevance and current trends
- Cell Cycle: Researchers commonly examine how CDC25C (M-phase inducer phosphatase 3) relates to this theme using model systems and orthogonal readouts.
- Epigenetics and Nuclear Signaling: Researchers commonly examine how CDC25C (M-phase inducer phosphatase 3) relates to this theme using model systems and orthogonal readouts.
- Kinases/Phosphatases: Researchers commonly examine how CDC25C (M-phase inducer phosphatase 3) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CDC25C (M-phase inducer phosphatase 3) levels across conditions; band patterns may reflect isoforms and processing.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
