| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ubiquitin-conjugating enzyme E2 R1;6.3.2.19;Ubiquitin-conjugating enzyme E2-32 kDa complementing;Ubiquitin-conjugating enzyme E2-CDC34;Ubiquitin-protein ligase R1;CDC34;UBCH3, UBE2R1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CDC34 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IBO-3 is a rabbit monoclonal antibody that recognizes ubiquitin-conjugating enzyme E2 R1 (gene CDC34) in human, mouse and rat samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 26.7 kDa, and the supplier reports an observed band at about 35 kDa.
CDC34 (UBE2R1) is an E2 ubiquitin-conjugating enzyme that works mainly with cullin-RING E3 ligases such as SCF complexes to build Lys48-linked ubiquitin chains. It targets cell cycle regulators, including the CDK inhibitor p27, for proteasomal degradation, linking it to G1/S progression.
| Target | Ubiquitin-conjugating enzyme E2 R1 (gene CDC34; UniProt P49427, human) |
|---|---|
| Clone | IBO-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 26.7 kDa |
| Observed MW | 35 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human 293T cells, human HepG2 cells, human K562 cells, human Caco-2 cells, human SH-SY5Y cells, human U251 cells, rat testis tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human CDC34.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ubiquitin-conjugating enzyme E2 R1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
