| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cell division cycle 5-like protein;Cdc5-like protein;Pombe cdc5-related protein;CDC5L;KIAA0432, PCDC5RP; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CDC5L, different from the related rat and mouse sequences by four amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CDC5L Antibody Picoband® is an antibody targeting CDC5L. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Human,Rat; observed MW: 100 kDa; calculated MW: 92251 MW.
Boster Bio Anti-CDC5L Antibody catalog # PA2123. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: CDC5L — Cell division cycle 5-like protein
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Human,Rat
- Molecular weight guidance: Observed: 100 kDa; Calculated: 92251 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR). .
Scientific background (datasheet): Cell division cycle 5-like protein, also called PCDC5RP or CDC5-like, is a protein that in humans is encoded by the CDC5L gene. The CDC5L gene was mapped to chromosome 6p21 by fluorescence in situ hybridization. This DNA-binding protein involved in cell cycle control. It may act as a transcription activator. The Component of the PRP19-CDC5L complex forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The role of CDC5 (Polo-like kinase) in regulating Rho1 is likely to be relevant to cytokinesis and asymmetric cell division in other organisms.
Cellular localization (datasheet): Nucleus . Nucleus speckle . Cytoplasm . May shuttle between cytoplasm and nucleus. .
Tissue details (datasheet): Ubiquitously expressed in both fetal and adult tissues. .
Sequence similarities (datasheet): Belongs to the CEF1 family.
Research relevance and current trends
- Commonly studied in contexts related to DNA/RNA,Epigenetics and Nuclear Signaling,RNA Processing,Splicing.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
