| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cell division control protein 6 homolog;CDC6-related protein;Cdc18-related protein;HsCdc18;p62 (cdc6);HsCDC6;CDC6;CDC18L; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Cdc6 recombinant protein (Position: V147-A549). Human Cdc6 shares 87.3% amino acid (aa) sequence identity with mouse Cdc6. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CDC6 (Cell division control protein 6 homolog) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Cdc6 Antibody Picoband® catalog # PB9531. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human Cdc6 recombinant protein (Position: V147-A549). Human Cdc6 shares 87.3% amino acid (aa) sequence identity with mouse Cdc6. (reported region: V147-A549).
- Molecular weight context: reported MW: 63 kDa; calculated MW: 62720 MW
- Reactivity: Human,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Cell division control protein 6 homolog; Cell division control protein 6 homolog. Cell division control ptotein 6 homolog, also called CDC18L or CDC6, is a protein that in humans is encoded by the CDC6 gene. By analysis of somatic cell hybrids and by fluorescence in situ hybridization, this gene is mapped to 17q21.2. The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. And this protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. This gene is involved in the initiation of DNA replication. Functional note: Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. Reported localization: Nucleus . Cytoplasm . The protein is nuclear in G1 and cytoplasmic in S-phase cells. Expression/tissue context: Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.
Research relevance and current trends
- Cell Cycle: Researchers commonly examine how CDC6 (Cell division control protein 6 homolog) relates to this theme using model systems and orthogonal readouts.
- Cell Division: Researchers commonly examine how CDC6 (Cell division control protein 6 homolog) relates to this theme using model systems and orthogonal readouts.
- DNA/RNA: Researchers commonly examine how CDC6 (Cell division control protein 6 homolog) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CDC6 (Cell division control protein 6 homolog) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CDC6 (Cell division control protein 6 homolog) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the CDC6/cdc18 family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
