| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin-dependent kinase 1; CDK1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDK1; CDC2P; CDC28A; CDKN1; P34CDC2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CDK1 recombinant protein (Position: L66-M297). Human CDK1 shares 97.8% and 98.3% amino acid (aa) sequence identity with mouse and rat CDK1, respectively. |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 2G11 is a mouse monoclonal antibody that recognizes cyclin-dependent kinase 1 (gene CDK1) in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 34.1 kDa, and the supplier reports an observed band at about 34 kDa.
CDK1 (CDC2) is the cyclin-dependent kinase that drives cells into mitosis. Bound mainly to cyclin B, it phosphorylates many substrates that trigger nuclear envelope breakdown, chromosome condensation and spindle assembly, and it also helps regulate centrosome duplication, making it a central marker and target in cell cycle and cancer research.
| Target | Cyclin-dependent kinase 1 (gene CDK1; UniProt P06493, human) |
|---|---|
| Clone | 2G11 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 34.1 kDa |
| Observed MW | 34 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): human HEK293 cells, human A549 cells, human HepG2 cells, human THP-1 cells, human PANC-1 cells, human SW620 cells, rat RH35 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, human tonsil tissue, human lung cancer tissue, mouse testis tissue, rat testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with a confirmed signal (flow cytometry): PC-3 cells, U2OS cells.
Immunogen
Recombinant human CDK1 fragment (Leu66–Met297), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 97.8% and 98.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cyclin-dependent kinase 1 in the nucleus, cytoplasm and mitochondrion. Tissue expression noted by UniProt (human): Found in breast cancer tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hanliang Sun et al. (2025) 5-Methylcytosine-modified circRNA-CCNL2 regulates vascular remdeling in hypoxic pulmonary hypertension through binding to FXR2. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.139638
Chunhui Yang et al. (2013) Alantolactone inhibits growth of K562/adriamycin cells by downregulating Bcr/Abl and P-glycoprotein expression. IUBMB Life. 10.1002/iub.1141
Fengmei Song et al. (2017) Pseudolaric acid B inhibits neuroglioma cell proliferation through DNA damage response. Oncology Reports. 10.3892/or.2017.5861
Junming Guo et al. (2009) Suppression of C-myc Expression Associates with Anti-Proliferation of Aloe-Emodin on Gastric Cancer Cells. Cancer Investigation. 10.1080/07357900701788130
