| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Carcinoembryonic antigen-related cell adhesion molecule 5; Carcinoembryonic antigen; CEA; Meconium antigen 100; CD66e; CEACAM5 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Carcinoembryonic antigen-related cell adhesion molecule 5 (CEA) isolated from a human colon adenocarcinoma cell line. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CEACAM5/Cd66e Monoclonal Antibody is an antibody targeting CEACAM1. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: C6G9; isotype: Mouse IgG1; reactivity: Human; observed MW: 37 kDa; calculated MW: 37141 MW.
Boster Bio Anti-CEACAM5/Cd66e Monoclonal Antibody catalog # MA1023. Tested in IHC, WB applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CEACAM1 — Carcinoembryonic antigen-related cell adhesion molecule 5
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: C6G9; Isotype: Mouse IgG1
- Species reactivity: Human
- Molecular weight guidance: Observed: 37 kDa; Calculated: 37141 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:2803308, PubMed:10910050, PubMed:10864933). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells
Scientific background (datasheet): Carcinoembryonic antigen is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.
Cellular localization (datasheet): Cell membrane
Tissue details (datasheet): Expressed in columnar epithelial and goblet cells of the colon (at protein level) (PubMed:10436421). Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
Sequence similarities (datasheet): Belongs to the immunoglobulin superfamily. CEA family.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cell Type Markers,Human Protein,Microbiology,Oncoproteins/Suppressors,Protein,Tags & Cell Markers,Tumor Antigens,Tumor Associated,Tumor Biomarkers,Tumor Immunology,Tumor Suppressors,Tumor-Associated Antigens.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
