Anti-CEBP Delta/CEBPD Antibody Picoband®

Overview

Anti-CEBP Delta/CEBPD Antibody Picoband® is an antibody for CEBPD detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: CEBPD (Calpain-2 catalytic subunit); UniProt: P49716
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 36 kDa, calculated 79995 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-CEBP Delta/CEBPD Antibody Picoband® catalog # A03499-4.

Biological background

Biological context: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). .

Expression and localization notes: cellular localization: Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca (2+) binding., tissue context: Ubiquitous..

Common research applications

• Western blotting (WB): Compare CEBPD levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of CEBPD in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify CEBPD-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: CCAAT/enhancer-binding protein delta is a protein that in humans is encoded by the CEBPD gene. The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-alpha. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses, and may be involved in the regulation of genes associated with activation and/or differentiation of macrophages. The cytogenetic location of this locus has been reported as both 8p11 and 8q11.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca (2+) binding.
• Tissue details: Ubiquitous.
• Research category: Calcium Signaling,Cancer,Cancer Metabolism,Cell Biology,Metabolism,Metabolism Processes,Pathways and Processes,Proteolysis/Ubiquitin,Proteolytic Enzymes,Response To Hypoxia,Signal Transduction,Signaling Pathway

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.