| Field | Specification |
|---|---|
| Target | |
| Alternative names | Histone H3-like centromeric protein A;Centromere autoantigen A;Centromere protein A;CENP-A;CENPA; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CENPA recombinant protein (Position: M1-G140). Human CENPA shares 71.6% amino acid (aa) sequence identity with mouse CENPA. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects histone H3-like centromeric protein A (gene CENPA) in human and rat samples and is validated for Western blot. The predicted molecular weight is 16.0 kDa, and the supplier reports an observed band at about 16 kDa.
CENP-A is a histone H3 variant that replaces canonical H3 in centromeric nucleosomes and epigenetically marks the site of the centromere. It serves as the foundation for kinetochore assembly, so it is essential for accurate chromosome segregation and is a key protein in chromosome biology research.
| Target | Histone H3-like centromeric protein A (gene CENPA; UniProt P49450, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 16.0 kDa |
| Observed MW | 16 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): rat liver tissue, rat lung tissue, rat pancreas tissue, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human CENPA fragment (Met1–Gly140), expressed in E. coli. Sequence identity with the mouse ortholog: 71.6%.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places histone H3-like centromeric protein A in the nucleus and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
