| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Concentration | |
| Formulation | |
| Immunogen | Affinity purified |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Shipping | |
| Storage |
Product Overview
Research Use OnlyMurine choline-O-acetyltransferase (EC 2.3.1.6) is a 71,721 dalton protein (640 amino acids) expressed in cholinergic neurons of both the PNS and CNS. In the CNS, ChAT is expressed in motor neurons and pre-ganglionic autonomic neurons of the spinal cord, a subset of neurons in the neostriatum, and in the basal forebrain. In the PNS, ChAT is expressed in a small subpopulation of sympathetic neurons and in all parasympathetic neurons.
Validated Applications
| Immunohistochemistry (IHC) | See datasheet for recommended dilution |
|---|---|
| Immunofluorescence (IF/ICC) | See datasheet for recommended dilution |
Key Specifications
| Clonality | Polyclonal |
|---|---|
| Species Reactivity | Human,Mouse |
| Isotype | IgY |
| Concentration | 1ug/ul |
| Formulation | Liquid |
| Catalog No. | G143 |
Immunogen / Antigen
Affinity purified
Reactivity Notes
An antipeptide antibody was generated in chickens against a sequence shared between the mouse (Q03059) and human (P28329) gene products.
Additional Information
Quality Note: abm guarantees that all our Anti-ChAT(Chicken) Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Do
Storage & Handling
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Immunohistochemistry, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
Recommended dilutions vary by application and sample type. Start with the manufacturer's suggested range (typically 1:500–1:2000 for WB; 1:50–1:200 for IHC/IF) and optimize for your specific experimental conditions.
This antibody has been confirmed reactive against: Human,Mouse. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Affinity purified
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
