| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Choline O-acetyltransferase;CHOACTase;ChAT;Choline acetylase;2.3.1.6;CHAT; |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CHAT recombinant protein (Position: D446-R652). Human CHAT shares 88.4% and 87% amino acid (aa) sequence identity with mouse and rat CHAT, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CHAT (Choline O-acetyltransferase) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Choline Acetyltransferase/CHAT Antibody Picoband® catalog # A01192-3. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human CHAT recombinant protein (Position: D446-R652). Human CHAT shares 88.4% and 87% amino acid (aa) sequence identity with mouse and rat CHAT, respectively. (reported region: D446-R652).
- Molecular weight context: reported MW: 83 kDa; calculated MW: 82536 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Choline O-acetyltransferase; Choline O-acetyltransferase. Choline acetyltransferase (commonly abbreviated as ChAT, but sometimes CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. In humans, the choline acetyltransferase enzyme is encoded by the CHAT gene. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. Functional note: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Research relevance and current trends
- Cell Type Marker: Researchers commonly examine how CHAT (Choline O-acetyltransferase) relates to this theme using model systems and orthogonal readouts.
- Neuron Marker: Researchers commonly examine how CHAT (Choline O-acetyltransferase) relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how CHAT (Choline O-acetyltransferase) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CHAT (Choline O-acetyltransferase) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CHAT (Choline O-acetyltransferase) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
