| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Transcription factor 7-like 1;HMG box transcription factor 3;TCF-3;TCF7L1;TCF3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human CHX10 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-VSX2 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 24V98; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, Flow (as provided in the source record). Boster Bio Anti-CHX10 Rabbit Monoclonal Antibody catalog # M05180. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: VSX2 (Transcription factor 7-like 1).
- Antibody format: Monoclonal; clone 24V98; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
VSX2 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis (By similarity). Down-regulates NQO1, leading to increased mitomycin c resistance. . Reported cellular localization context: Nucleus. Tissue expression notes (as provided): Detected in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium. .
Research relevance and current trends
- Research context keywords from the source record include: Growth Factors/Hormones,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate VSX2 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect VSX2 expression by Western blot in cell or tissue lysates, Quantify VSX2-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 50 kDa; calculated MW: 62631 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 50 kDa
- Cellular localization (provided): Nucleus.
- Tissue details (provided): Detected in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
