| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein capicua homolog; CIC; KIAA0306 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human CIC recombinant protein (Position: E1433-Q1527). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets protein capicua homolog (gene CIC) in human, mouse and rat samples and is validated for ELISA and IHC. The predicted molecular weight is 163.8 kDa.
Capicua (CIC) is an HMG-box transcriptional repressor that keeps genes downstream of receptor tyrosine kinase-RAS-MAPK signaling switched off until the pathway is active. It works with ATXN1 in brain development, and CIC alterations are well described in oligodendroglioma and CIC-rearranged sarcomas.
| Target | Protein capicua homolog (gene CIC; UniProt Q96RK0, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 163.8 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with confirmed staining (IHC): human brain tissue, human gastric cancer tissue, human placenta tissue, mouse lung tissue, rat intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant human CIC fragment (Glu1433–Gln1527), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein capicua homolog in the nucleus. Tissue expression noted by UniProt (human): Expressed in fetal brain.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xi Jin et al. (2013) Mechanisms underlying the beneficial effects of Kaiyu Granule for depression. Neural Regeneration Research. 10.3969/j.issn.1673-5374.2013.34.008