| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Sodium-dependent phosphate transport protein 2B; Sodium-phosphate transport protein 2B; Na (+)-dependent phosphate cotransporter 2B; NaPi3b; Sodium/phosphate cotransporter 2B; Na (+)/Pi cotransporter 2B; NaPi-2b; Solute carrier family 34 member 2; SLC34A2 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Cip4/TRIP10 recombinant protein (Position: M1-M182). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Cip4/TRIP10 Antibody Picoband® is an antibody for TRIP10 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: TRIP10 (solute carrier family 34 member 2); UniProt: Q15642
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 85 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Cip4/TRIP10 Antibody Picoband® catalog # A03963-1.
Biological background
Biological context: May be involved in actively transporting phosphate into cells via Na+ cotransport. It may be the main phosphate transport protein in the intestinal brush border membrane. May have a role in the synthesis of surfactant in lungs' alveoli.
Expression and localization notes: cellular localization: Membrane. Multi-pass membrane protein., tissue context: Highly expressed in lung. Also detected in pancreas, kidney, small intestine, ovary, testis, prostate and mammary gland. In lung, it is found in alveolar type II cells but not in bronchiolar epithelium..
Common research applications
- Western blotting (WB): Compare TRIP10 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of TRIP10 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify TRIP10-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Cdc42-interacting protein 4 is a protein that in humans is encoded by the TRIP10 gene. TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a (CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2). Western blot analysis usually demonstrates a major band around 75 kDa and a weaker band about 85 kDa.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Membrane. Multi-pass membrane protein.
- Tissue details: Highly expressed in lung. Also detected in pancreas, kidney, small intestine, ovary, testis, prostate and mammary gland. In lung, it is found in alveolar type II cells but not in bronchiolar epithelium.
- Research category: Channels,Metabolism,Plasma Membrane,Signal Transduction,Vitamins/Minerals
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
