| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cdc42-interacting protein 4; Protein Felic; Salt tolerant protein; hSTP; Thyroid receptor-interacting protein 10; TR-interacting protein 10; TRIP-10; TRIP10; CIP4; STOT; STP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Cip4/TRIP10 recombinant protein (Position: M1-M182). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets cdc42-interacting protein 4 (gene TRIP10) in human and rat samples and is validated for Western blot, ICC/IF and ELISA. The predicted molecular weight is 68.4 kDa, and the supplier reports an observed band at about 85 kDa.
CIP4 (TRIP10) is an F-BAR domain protein that binds membranes and the GTPase CDC42 and links membrane bending to actin assembly through WASP family proteins. It promotes membrane tubulation during endocytosis and supports insulin-stimulated GLUT4 translocation, so it is studied in endocytosis, cell migration and glucose uptake.
| Target | Cdc42-interacting protein 4 (gene TRIP10; UniProt Q15642, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 68.4 kDa |
| Observed MW | 85 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human HeLa cells, rat testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A431 cells.
Immunogen
Recombinant human Cip4/TRIP10 fragment (Met1–Met182), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places cdc42-interacting protein 4 in the cytoplasm, lysosome, Golgi apparatus and cell membrane. Tissue expression noted by UniProt (human): Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
