Anti-CISD2 Antibody Picoband®

Overview

Anti-CISD2 Antibody Picoband® is an antibody for CISD2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Monkey,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: CISD2 (Rho-related GTP-binding protein Rho6); UniProt: Q8N5K1
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 15 kDa, calculated 26056 MW
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-CISD2 Antibody Picoband® catalog # A06387-1.

Biological background

Biological context: Lacks intrinsic GTPase activity. Has a low affinity for GDP, and constitutively binds GTP. Controls rearrangements of the actin cytoskeleton. Induces the Rac-dependent neuritic process formation in part by disruption of the cortical actin filaments. Causes the formation of many neuritic processes from the cell body with disruption of the cortical actin filaments. .

Expression and localization notes: cellular localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton ., tissue context: Mostly expressed in brain and liver..

Common research applications

• Western blotting (WB): Compare CISD2 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of CISD2 in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify CISD2-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: CDGSH iron sulfur domain 2 is a protein that in humans is encoded by the CISD2 gene. The protein encoded by this gene is a zinc finger protein that localizes to the endoplasmic reticulum. The encoded protein binds an iron/sulfur cluster and may be involved in calcium homeostasis. Defects in this gene are a cause of Wolfram syndrome 2.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton .
• Tissue details: Mostly expressed in brain and liver.
• Research category: Actin Assembly,Actin, etc.,Cytoskeleton,Cytoskeleton/ECM,G Protein Signaling,Microfilaments,Ras Family,Signal Transduction,Signaling Pathway,Small G Proteins

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.