| Field | Specification |
|---|---|
| Target | |
| Alternative names | CDGSH iron-sulfur domain-containing protein 2; Endoplasmic reticulum intermembrane small protein; MitoNEET-related 1 protein; Miner1; Nutrient-deprivation autophagy factor-1; NAF-1; CISD2; CDGSH2; ERIS; ZCD2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CISD2 recombinant protein (Position: A55-N124). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets CDGSH iron-sulfur domain-containing protein 2 (gene CISD2) in human, monkey, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 15.3 kDa, and the supplier reports an observed band at about 15 kDa.
CISD2 (NAF-1) is an iron-sulfur protein located at the endoplasmic reticulum and mitochondrial membranes. It supports BCL2 in restraining Beclin 1-dependent autophagy and helps maintain calcium and mitochondrial homeostasis; loss of CISD2 causes Wolfram syndrome type 2, and it is studied in aging research.
| Target | CDGSH iron-sulfur domain-containing protein 2 (gene CISD2; UniProt Q8N5K1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 15.3 kDa |
| Observed MW | 15 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat, Monkey) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEK293 cells, human HeLa cells, human MCF-7 cells, monkey kidney tissue, human SW620 cells, human Raji cells, rat kidney tissue, mouse kidney tissue, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Recombinant human CISD2 fragment (Ala55–Asn124), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places CDGSH iron-sulfur domain-containing protein 2 in the endoplasmic reticulum membrane and mitochondrion outer membrane. Tissue expression noted by UniProt (human): Testis, small intestine, kidney, lung, brain, heart, pancreas and platelets. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
