| Field | Specification |
|---|---|
| Target | |
| Alternative names | Electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial; Calcium-binding mitochondrial carrier protein Aralar2; ARALAR-related gene 2; ARALAR2; Citrin; Mitochondrial aspartate glutamate carrier 2; Solute carrier family 25 member 13; SLC25A13; AGC2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Citrin/SLC25A13 recombinant protein (Position: M1-A643). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial in human and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 74.2 kDa, and the supplier reports an observed band at about 70 kDa.
Citrin (AGC2, SLC25A13) is a Ca2+-regulated mitochondrial aspartate–glutamate carrier expressed mainly in the liver. It supplies cytosolic aspartate for the urea cycle and supports the malate–aspartate shuttle, and its deficiency causes citrullinemia type II and neonatal intrahepatic cholestasis.
| Target | Electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial (gene SLC25A13; UniProt Q9UJS0, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 74.2 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Raji cells, human A431 cells, human 293T cells, human HepG2 cells, rat kidney tissue, rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human appendix adenocarcinoma tissue, human breast cancer tissue, human colon adenocarcinoma tissue, human diffuse large B cells lymphoma tissue, human glioblastoma tissue, human liver cancer tissue, human lung adenocarcinoma tissue, human placenta tissue, human prostate adenocarcinoma tissue, human right renal oncocytoma tissue, human thyroid papillary carcinoma tissue, human urothelial carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with confirmed staining (ICC/IF): human liver cancer tissue, human colon cancer tissue.
Samples with a confirmed signal (flow cytometry): Jurkat cells, U87 cells.
Immunogen
Recombinant human Citrin/SLC25A13 fragment (Met1–Ala643), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial in the mitochondrion inner membrane. Tissue expression noted by UniProt (human): High levels in liver and low levels in kidney, pancreas, placenta, heart and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
